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bio beads s x3 gel permeation chromatography column  (Bio-Rad)


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    Bio-Rad bio beads s x3 gel permeation chromatography column
    Bio Beads S X3 Gel Permeation Chromatography Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 405 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bio+gel+p+30+gel+permeation+chromatography/Bio-Gel+P-30+Gel/pm40158676-77-20-30
    Average 95 stars, based on 405 article reviews
    bio beads s x3 gel permeation chromatography column - by Bioz Stars, 2026-09
    95/100 stars

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    GPC Assay:

    Article Title: Paneth Cell α-Defensins from Rhesus Macaque Small Intestine
    Article Snippet: Small intestinal proteins were extracted from a sample of rhesus macaque jejunum homogenized in 30% acetic acid and clarified by ultracentrifugation at 25,000 rpm for 2 h in an SW28 rotor (Beckman Instruments, Fullerton, Calif.) at 4°C (1, 20, 32). .. Supernatants were separated by Bio-Gel P-30 gel permeation chromatography (BioRad, Hercules, Calif.) with 5% acetic acid, and 0.5- l samples of column fractions were mixed with 0.5 l of -cyano-4-hydroxycinnamic acid (10 mg/ml) on an Applied Biosystems V700666 sample plate and analyzed by matrix-assisted laser desorption ionization– time-of-flight mass spectrometry (MALDI-TOF MS) with a Voyager-DE STR instrument (Applied Biosystems) in the Physical Sciences Mass Spectrometry Facility at the University of California, Irvine. ..

    Article Title: Paneth Cell ?-Defensins from Rhesus Macaque Small Intestine
    Article Snippet: Small intestinal proteins were extracted from a sample of rhesus macaque jejunum homogenized in 30% acetic acid and clarified by ultracentrifugation at 25,000 rpm for 2 h in an SW28 rotor (Beckman Instruments, Fullerton, Calif.) at 4°C ( 1 , 20 , 32 ). .. Supernatants were separated by Bio-Gel P-30 gel permeation chromatography (Bio-Rad, Hercules, Calif.) with 5% acetic acid, and 0.5-μl samples of column fractions were mixed with 0.5 μl of α-cyano-4-hydroxycinnamic acid (10 mg/ml) on an Applied Biosystems V700666 sample plate and analyzed by matrix-assisted laser desorption ionization- time-of-flight mass spectrometry (MALDI-TOF MS) with a Voyager-DE STR instrument (Applied Biosystems) in the Physical Sciences Mass Spectrometry Facility at the University of California, Irvine. ..

    Mass Spectrometry:

    Article Title: Paneth Cell α-Defensins from Rhesus Macaque Small Intestine
    Article Snippet: Small intestinal proteins were extracted from a sample of rhesus macaque jejunum homogenized in 30% acetic acid and clarified by ultracentrifugation at 25,000 rpm for 2 h in an SW28 rotor (Beckman Instruments, Fullerton, Calif.) at 4°C (1, 20, 32). .. Supernatants were separated by Bio-Gel P-30 gel permeation chromatography (BioRad, Hercules, Calif.) with 5% acetic acid, and 0.5- l samples of column fractions were mixed with 0.5 l of -cyano-4-hydroxycinnamic acid (10 mg/ml) on an Applied Biosystems V700666 sample plate and analyzed by matrix-assisted laser desorption ionization– time-of-flight mass spectrometry (MALDI-TOF MS) with a Voyager-DE STR instrument (Applied Biosystems) in the Physical Sciences Mass Spectrometry Facility at the University of California, Irvine. ..

    Article Title: Paneth Cell ?-Defensins from Rhesus Macaque Small Intestine
    Article Snippet: Small intestinal proteins were extracted from a sample of rhesus macaque jejunum homogenized in 30% acetic acid and clarified by ultracentrifugation at 25,000 rpm for 2 h in an SW28 rotor (Beckman Instruments, Fullerton, Calif.) at 4°C ( 1 , 20 , 32 ). .. Supernatants were separated by Bio-Gel P-30 gel permeation chromatography (Bio-Rad, Hercules, Calif.) with 5% acetic acid, and 0.5-μl samples of column fractions were mixed with 0.5 μl of α-cyano-4-hydroxycinnamic acid (10 mg/ml) on an Applied Biosystems V700666 sample plate and analyzed by matrix-assisted laser desorption ionization- time-of-flight mass spectrometry (MALDI-TOF MS) with a Voyager-DE STR instrument (Applied Biosystems) in the Physical Sciences Mass Spectrometry Facility at the University of California, Irvine. ..



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    Figure 1 Calibration of Superdex Peptide HR10/30 gel permeation chromatography column by using standard peptides and the elution profile of the hydrophilic fraction of soy crude peptide-LD3.

    Journal: Lipids in Health and Disease

    Article Title: Screening of soy protein-derived hypotriglyceridemic di-peptides in vitro and in vivo

    doi: 10.1186/1476-511x-10-85

    Figure Lengend Snippet: Figure 1 Calibration of Superdex Peptide HR10/30 gel permeation chromatography column by using standard peptides and the elution profile of the hydrophilic fraction of soy crude peptide-LD3.

    Article Snippet: Hydrophilic fraction was further fractionated by gel permeation chromatography-high performance liquid chromatography (GPC-HPLC, Bio-Rad Superdex Peptide HR10/30 column, 30% CH3CN/0.1% trifluoroacetic acid (TFA), 0.3 mL/min), and di- or tri-peptide fractions were collected at an elution time ranging from 45 to 75 min (Figure 1; Fraction A, Frc-A, 45-55 min; Fraction B, Frc-B, 55-65 min; Fraction C, Frc-C, 65-75 min).

    Techniques: GPC Assay